The panel was published in the 143rd chapter of the JoJo’s Bizarre Adventure: Stardust Crusaders manga series on February 24th, 1992.
The scene was also featured in the Stardust Crusaders anime episode 46th, titled Dio’s World, Part 2, which was aired on June 5th, 2015.
Edits of the comic book panel first started appearing, shortly after the anime episode was aired.
One of the first posts appeared on Tumblr on June 13th, 2015.
“Oh you’re approaching me” had seen a lot of variations, as the meme appeared on sites such as Reddit, Imgur as well as Twitter.